Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod

Rahel Tilahun

doi:doi:10.11648/j.ijmb.20200504.14

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Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod

Rahel Tilahun

Published in International Journal of Microbiology and Biotechnology (Volume 5, Issue 4)

Received: 19 November 2020 Accepted: 3 December 2020 Published: 16 December 2020

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Abstract

At present alkaline proteases are widely used in the detergent, leather tanning, pharmaceutical, food, and feed processing industries. Although proteases are found in all living organisms (plant, animal, and microorganisms), the bulk of commercially important enzymes are from microorganisms. The aim of this study was to isolate protease producing bacteria, characterize the enzyme, and evaluate potential application as detergent additive to enhance the washing performance of endod berries. Different bacterial strains were isolated from soil and screened using an alkaline casein agar media. One isolate designated as aau 2106 was selected for further study because of its high proteoliytic activity, level of enzyme production and, ability to grow under solid state fermentation (SSF). Cultivation condition for the production of the enzyme under SSF, such as, moisture content, nitrogen content, and incubation period were optimized. The enzyme was active in the pH range of 6 to 10.5, temperature of 40 to 75°C. And optimum in; 96h incubation period, 1:1 ratio of moisture content and, casein in the production of enzyme under SSF and the enzyme was stable in the presence of endod and commercial detergents. The potential of the enzyme as a detergent additive was tested by adding the protease (57 U/g) in detergent formulations and used to clean pieces of cloth stained with blood and egg. Addition of the enzyme improved the cleaning efficiency of both endod and commercial detergents. This indicates that enzyme supplementation of endod could result in the development of an ecofriendly detergent.

Published in	International Journal of Microbiology and Biotechnology (Volume 5, Issue 4)
DOI	10.11648/j.ijmb.20200504.14
Page(s)	193-202
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Detergent, Endod, Protease

References

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[17]	Banerjee, U. C., Sani, R. K., Azmi, W., and Soni, R. (1999). Thermostable alkaline protease from Bacillus brevis and its characterization as laundry detergent additive. Process Biochemistry. pp. 35: 213-19.
[18]	Hameed, A., Keshavarz, T. and Evans, C. S. (1999). Effect of dissolved oxygen tension and pH on the production of extracellular protease from a new isolate of Bacillus subtilisK2 for use in leather processing. Chemical Technology and Biotechnology. pp. 74: 5-8.
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APA Style

Rahel Tilahun. (2020). Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod. International Journal of Microbiology and Biotechnology, 5(4), 193-202. https://doi.org/10.11648/j.ijmb.20200504.14

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ACS Style

Rahel Tilahun. Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod. Int. J. Microbiol. Biotechnol. 2020, 5(4), 193-202. doi: 10.11648/j.ijmb.20200504.14

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AMA Style

Rahel Tilahun. Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod. Int J Microbiol Biotechnol. 2020;5(4):193-202. doi: 10.11648/j.ijmb.20200504.14

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@article{10.11648/j.ijmb.20200504.14,
  author = {Rahel Tilahun},
  title = {Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {4},
  pages = {193-202},
  doi = {10.11648/j.ijmb.20200504.14},
  url = {https://doi.org/10.11648/j.ijmb.20200504.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200504.14},
  abstract = {At present alkaline proteases are widely used in the detergent, leather tanning, pharmaceutical, food, and feed processing industries. Although proteases are found in all living organisms (plant, animal, and microorganisms), the bulk of commercially important enzymes are from microorganisms. The aim of this study was to isolate protease producing bacteria, characterize the enzyme, and evaluate potential application as detergent additive to enhance the washing performance of endod berries. Different bacterial strains were isolated from soil and screened using an alkaline casein agar media. One isolate designated as aau 2106 was selected for further study because of its high proteoliytic activity, level of enzyme production and, ability to grow under solid state fermentation (SSF). Cultivation condition for the production of the enzyme under SSF, such as, moisture content, nitrogen content, and incubation period were optimized. The enzyme was active in the pH range of 6 to 10.5, temperature of 40 to 75°C. And optimum in; 96h incubation period, 1:1 ratio of moisture content and, casein in the production of enzyme under SSF and the enzyme was stable in the presence of endod and commercial detergents. The potential of the enzyme as a detergent additive was tested by adding the protease (57 U/g) in detergent formulations and used to clean pieces of cloth stained with blood and egg. Addition of the enzyme improved the cleaning efficiency of both endod and commercial detergents. This indicates that enzyme supplementation of endod could result in the development of an ecofriendly detergent.},
 year = {2020}
}

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TY  - JOUR
T1  - Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod
AU  - Rahel Tilahun
Y1  - 2020/12/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200504.14
DO  - 10.11648/j.ijmb.20200504.14
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 193
EP  - 202
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200504.14
AB  - At present alkaline proteases are widely used in the detergent, leather tanning, pharmaceutical, food, and feed processing industries. Although proteases are found in all living organisms (plant, animal, and microorganisms), the bulk of commercially important enzymes are from microorganisms. The aim of this study was to isolate protease producing bacteria, characterize the enzyme, and evaluate potential application as detergent additive to enhance the washing performance of endod berries. Different bacterial strains were isolated from soil and screened using an alkaline casein agar media. One isolate designated as aau 2106 was selected for further study because of its high proteoliytic activity, level of enzyme production and, ability to grow under solid state fermentation (SSF). Cultivation condition for the production of the enzyme under SSF, such as, moisture content, nitrogen content, and incubation period were optimized. The enzyme was active in the pH range of 6 to 10.5, temperature of 40 to 75°C. And optimum in; 96h incubation period, 1:1 ratio of moisture content and, casein in the production of enzyme under SSF and the enzyme was stable in the presence of endod and commercial detergents. The potential of the enzyme as a detergent additive was tested by adding the protease (57 U/g) in detergent formulations and used to clean pieces of cloth stained with blood and egg. Addition of the enzyme improved the cleaning efficiency of both endod and commercial detergents. This indicates that enzyme supplementation of endod could result in the development of an ecofriendly detergent.
VL  - 5
IS  - 4
ER  -

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Author Information

Rahel Tilahun

School of Graduate Studies, Microbial, Cellular, and Molecular Biology Department, Addis Ababa University, Addis Ababa, Ethiopia

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APA Style

Rahel Tilahun. (2020). Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod. International Journal of Microbiology and Biotechnology, 5(4), 193-202. https://doi.org/10.11648/j.ijmb.20200504.14

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ACS Style

Rahel Tilahun. Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod. Int. J. Microbiol. Biotechnol. 2020, 5(4), 193-202. doi: 10.11648/j.ijmb.20200504.14

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AMA Style

Rahel Tilahun. Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod. Int J Microbiol Biotechnol. 2020;5(4):193-202. doi: 10.11648/j.ijmb.20200504.14

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@article{10.11648/j.ijmb.20200504.14,
  author = {Rahel Tilahun},
  title = {Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {4},
  pages = {193-202},
  doi = {10.11648/j.ijmb.20200504.14},
  url = {https://doi.org/10.11648/j.ijmb.20200504.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200504.14},
  abstract = {At present alkaline proteases are widely used in the detergent, leather tanning, pharmaceutical, food, and feed processing industries. Although proteases are found in all living organisms (plant, animal, and microorganisms), the bulk of commercially important enzymes are from microorganisms. The aim of this study was to isolate protease producing bacteria, characterize the enzyme, and evaluate potential application as detergent additive to enhance the washing performance of endod berries. Different bacterial strains were isolated from soil and screened using an alkaline casein agar media. One isolate designated as aau 2106 was selected for further study because of its high proteoliytic activity, level of enzyme production and, ability to grow under solid state fermentation (SSF). Cultivation condition for the production of the enzyme under SSF, such as, moisture content, nitrogen content, and incubation period were optimized. The enzyme was active in the pH range of 6 to 10.5, temperature of 40 to 75°C. And optimum in; 96h incubation period, 1:1 ratio of moisture content and, casein in the production of enzyme under SSF and the enzyme was stable in the presence of endod and commercial detergents. The potential of the enzyme as a detergent additive was tested by adding the protease (57 U/g) in detergent formulations and used to clean pieces of cloth stained with blood and egg. Addition of the enzyme improved the cleaning efficiency of both endod and commercial detergents. This indicates that enzyme supplementation of endod could result in the development of an ecofriendly detergent.},
 year = {2020}
}

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TY  - JOUR
T1  - Production, Characterization and Evaluation of Bacterial Protease as a Potential Additive to Enhance Detergency of Endod
AU  - Rahel Tilahun
Y1  - 2020/12/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200504.14
DO  - 10.11648/j.ijmb.20200504.14
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 193
EP  - 202
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200504.14
AB  - At present alkaline proteases are widely used in the detergent, leather tanning, pharmaceutical, food, and feed processing industries. Although proteases are found in all living organisms (plant, animal, and microorganisms), the bulk of commercially important enzymes are from microorganisms. The aim of this study was to isolate protease producing bacteria, characterize the enzyme, and evaluate potential application as detergent additive to enhance the washing performance of endod berries. Different bacterial strains were isolated from soil and screened using an alkaline casein agar media. One isolate designated as aau 2106 was selected for further study because of its high proteoliytic activity, level of enzyme production and, ability to grow under solid state fermentation (SSF). Cultivation condition for the production of the enzyme under SSF, such as, moisture content, nitrogen content, and incubation period were optimized. The enzyme was active in the pH range of 6 to 10.5, temperature of 40 to 75°C. And optimum in; 96h incubation period, 1:1 ratio of moisture content and, casein in the production of enzyme under SSF and the enzyme was stable in the presence of endod and commercial detergents. The potential of the enzyme as a detergent additive was tested by adding the protease (57 U/g) in detergent formulations and used to clean pieces of cloth stained with blood and egg. Addition of the enzyme improved the cleaning efficiency of both endod and commercial detergents. This indicates that enzyme supplementation of endod could result in the development of an ecofriendly detergent.
VL  - 5
IS  - 4
ER  -

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