Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI

Mahbuba Sultana; Akram Hossain; Shyamal Kumar Paul; Nobumichi Kobayashi; Md. Sharif Hossain; Mst. Nazma khatun; Mahbubul Alam

doi:doi:10.11648/j.ijmb.20210604.13

Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI

Mahbuba Sultana, Akram Hossain, Shyamal Kumar Paul, Nobumichi Kobayashi, Md. Sharif Hossain, Mst. Nazma khatun, Mahbubul Alam

Published in International Journal of Microbiology and Biotechnology (Volume 6, Issue 4)

Received: 9 October 2021 Accepted: 25 October 2021 Published: 10 November 2021

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Abstract

Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.

Published in	International Journal of Microbiology and Biotechnology (Volume 6, Issue 4)
DOI	10.11648/j.ijmb.20210604.13
Page(s)	119-124
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Major Uropathogens, Different Laboratory Diagnostic, Polymerase Chain Reaction (PCR)

References

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[4]	Zorc, J. J., Kiddoo, D. A. and Shaw, K. N. (2005) 'Diagnosis and Management of Pediatric Urinary Tract Infections Clinical Microbiology Reviews Vol- 18: 417-422.
[5]	Padmavaty, B., Kumar, R, V., Patel, A., Swarnam, S, D., Vaidehi, T., Ali, B. MJ (2012) "Rapid and sensitive Detection of Major Uropathogens in a Single - Pot Multiplex PCR Assay.' Vol. 65: 44-53.
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[7]	Masud MR, Afroz H, Fakruddin M. Prevalence of extended-spectrum β- lactamase positive bacteria in radiologically positive urinary tract infection. Springerplus. 2014; 3: 216. Published 2014 May 1. Doi: 10.1186/2193- 1801-3-216.
[8]	Ahmed, B., Akhter, M., Hasan, M., Alam, MK. (2011) Sensitivity patteren of urinary tract pathogens to antimicrobial drugs at a tertiary level hospital in Bangladesh'. Vol 17 (01): 18-21.
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[13]	Aeddula NR, Baradhi KM. Reflux Nephropathy. [Updated 2021 Jul 26]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK526055/.
[14]	Lalkhen, A. G. and McCluskey, A. (2008). Clinical tests: Sensitivity and specificity, continuing education in anaesthesia'. Critical Care and Pain. Vol. 8: 221-223.
[15]	Leblebicioglu, I and Esen, S. (2001) Nosocomial UFI in Turkey a nationwide multicenter point prevalence studyi, Intersei conf antimicrobial agentis chemother. Vol. 41: 6-9.
[16]	Aminu, K. Y. Aliyu, U. U. (2015) 'Asymptomatic bacteriuria in pregnant women in the Antenatal Booking Clinic at Aminu Kano Teaching Hospital, Kano, Nigeria'. Vol. 5: No. 5 DOI: 10.4236/o jog.
[17]	Moshaver, B., Boer, F. D., Kreileman, H. V., Kramer, E. Stegeman, C., Paul, G. (2016) 'Fast and accurate prediction of positive and negative urine culture by flow cytrometry'. Vol. 16 (1): 211.
[18]	Chowdhury, A. 1998) Urinary tract infection in pregnancy: a bacteriological study'. M phil (Microbiology) Thesis, Bangabandhu Sheikh Mujib Medical University, Bangladesh. P: 2-101.
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Mahbuba Sultana, Akram Hossain, Shyamal Kumar Paul, Nobumichi Kobayashi, Md. Sharif Hossain, et al. (2021). Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. International Journal of Microbiology and Biotechnology, 6(4), 119-124. https://doi.org/10.11648/j.ijmb.20210604.13

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ACS Style

Mahbuba Sultana; Akram Hossain; Shyamal Kumar Paul; Nobumichi Kobayashi; Md. Sharif Hossain, et al. Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. Int. J. Microbiol. Biotechnol. 2021, 6(4), 119-124. doi: 10.11648/j.ijmb.20210604.13

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AMA Style

Mahbuba Sultana, Akram Hossain, Shyamal Kumar Paul, Nobumichi Kobayashi, Md. Sharif Hossain, et al. Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. Int J Microbiol Biotechnol. 2021;6(4):119-124. doi: 10.11648/j.ijmb.20210604.13

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@article{10.11648/j.ijmb.20210604.13,
  author = {Mahbuba Sultana and Akram Hossain and Shyamal Kumar Paul and Nobumichi Kobayashi and Md. Sharif Hossain and Mst. Nazma khatun and Mahbubul Alam},
  title = {Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {6},
  number = {4},
  pages = {119-124},
  doi = {10.11648/j.ijmb.20210604.13},
  url = {https://doi.org/10.11648/j.ijmb.20210604.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210604.13},
  abstract = {Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.},
 year = {2021}
}

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TY  - JOUR
T1  - Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI
AU  - Mahbuba Sultana
AU  - Akram Hossain
AU  - Shyamal Kumar Paul
AU  - Nobumichi Kobayashi
AU  - Md. Sharif Hossain
AU  - Mst. Nazma khatun
AU  - Mahbubul Alam
Y1  - 2021/11/10
PY  - 2021
N1  - https://doi.org/10.11648/j.ijmb.20210604.13
DO  - 10.11648/j.ijmb.20210604.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 119
EP  - 124
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20210604.13
AB  - Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.
VL  - 6
IS  - 4
ER  -

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Author Information

Mahbuba Sultana

Department of Microbiology, Shaheed Tajuddin Ahmed Medical College, Gazipur, Bangladesh
Akram Hossain

Microbiology, Department of Laboratory Medicine, Imperial Hospital, Chattogram, Bangladesh
Shyamal Kumar Paul

Microbiology, Netrokona Medical College, Netrokona, Bangladesh
Nobumichi Kobayashi

Department of Hygiene, Sapporo Medical University, School of Medicine, Sapporo, Japan
Md. Sharif Hossain

Department of Orthopedics, Shaheed Tajuddin Ahmed Medical College Hospital, Gazipur, Bangladesh
Mst. Nazma khatun

Department of Microbiology, Shaheed Tajuddin Ahmed Medical College, Gazipur, Bangladesh
Mahbubul Alam

Department of Microbiology, Shaheed Tajuddin Ahmed Medical College, Gazipur, Bangladesh

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APA Style

Mahbuba Sultana, Akram Hossain, Shyamal Kumar Paul, Nobumichi Kobayashi, Md. Sharif Hossain, et al. (2021). Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. International Journal of Microbiology and Biotechnology, 6(4), 119-124. https://doi.org/10.11648/j.ijmb.20210604.13

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ACS Style

Mahbuba Sultana; Akram Hossain; Shyamal Kumar Paul; Nobumichi Kobayashi; Md. Sharif Hossain, et al. Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. Int. J. Microbiol. Biotechnol. 2021, 6(4), 119-124. doi: 10.11648/j.ijmb.20210604.13

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AMA Style

Mahbuba Sultana, Akram Hossain, Shyamal Kumar Paul, Nobumichi Kobayashi, Md. Sharif Hossain, et al. Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI. Int J Microbiol Biotechnol. 2021;6(4):119-124. doi: 10.11648/j.ijmb.20210604.13

Copy | Download

@article{10.11648/j.ijmb.20210604.13,
  author = {Mahbuba Sultana and Akram Hossain and Shyamal Kumar Paul and Nobumichi Kobayashi and Md. Sharif Hossain and Mst. Nazma khatun and Mahbubul Alam},
  title = {Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {6},
  number = {4},
  pages = {119-124},
  doi = {10.11648/j.ijmb.20210604.13},
  url = {https://doi.org/10.11648/j.ijmb.20210604.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210604.13},
  abstract = {Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Different Laboratory Diagnostic Procedures Including PCR Directly from Urine Specimens from Suspected Patients of UTI
AU  - Mahbuba Sultana
AU  - Akram Hossain
AU  - Shyamal Kumar Paul
AU  - Nobumichi Kobayashi
AU  - Md. Sharif Hossain
AU  - Mst. Nazma khatun
AU  - Mahbubul Alam
Y1  - 2021/11/10
PY  - 2021
N1  - https://doi.org/10.11648/j.ijmb.20210604.13
DO  - 10.11648/j.ijmb.20210604.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 119
EP  - 124
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20210604.13
AB  - Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Hence a study was necessary to apply PCR technology directly to clinical specimens to allow early and accurate identification of pathogens of UTI. Objective: Diagnosis of major uropathogens by different laboratory diagnostic procedures including polymerase chain reaction (PCR) directly from urine specimens from suspected patients of urinary tract infection. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolation and identification of major uropathogens (Escherichiacoli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure anbiochemical tests. PCR was performed by using standard protocol with species specific primer for detection offim H gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreC for Proteus mirabilis and ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same time PCR could detect species specific genes in 201 specimens directly from urine of suspected UTI patient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department. Urine specimens was higher in female in both out patient population and inpatient population. Culture positivity of in patient population among the male (45.5%) was slighty higher than that of outpatient population (34.5%). The predominant age group suffered from UTI in case of outpatient population was >15-30 but for the in patient population, the age group was 60 years and above. 90 culture positive in patient population, 43 (95.5%) were from the gynae unit, 23 (92%) were from surgery unit, 8 (72.7%) were from the medicine unit, 12 (85.7%) were from orthopaedic unit and 4 (80%) were from paediatric unit. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.
VL  - 6
IS  - 4
ER  -

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