Nematodes are one of the most economically important pests affecting banana production worldwide. Studies on how nematodes affect banana production involve isolating the nematodes from the banana fields for further analysis laboratory. Different protocols have been developed however, the protocols have not been fine-tuned to save on time and increase efficiency. The time required for isolation therefore needs to be optimized so that the optimal number of nematodes is isolated. This study reports optimization of nematodes isolation time from soil samples in a tissue culture banana plantation at 5 and 9 months. The nematodes harvesting experiments were set at different times (12, 20, 24, 30, 35 and 48 hours). It also reports the influence of harvesting time at 5 and 9 months when bananas were growing in the field. The study recorded the highest number of nematodes at 48 hours which was significantly different from all the other hours evaluated. It also found that harvesting time trends at 5 and 9 months did not have significant differences. The results imply that laboratories isolating nematodes from soils where tissue culture bananas are planted can use 48 hours as optimal time for extraction of nematodes.
| Published in | International Journal of Microbiology and Biotechnology (Volume 7, Issue 3) |
| DOI | 10.11648/j.ijmb.20220703.11 |
| Page(s) | 115-117 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Nematodes, Tissue Culture Bananas, Optimum Nematodes Isolation Time
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APA Style
Dancun King’ang’i Muchira, Elias Mwangi, Richard Odour. (2022). Optimized Nematodes Isolation Protocol for Soils from Tissue Culture Banana Field. International Journal of Microbiology and Biotechnology, 7(3), 115-117. https://doi.org/10.11648/j.ijmb.20220703.11
ACS Style
Dancun King’ang’i Muchira; Elias Mwangi; Richard Odour. Optimized Nematodes Isolation Protocol for Soils from Tissue Culture Banana Field. Int. J. Microbiol. Biotechnol. 2022, 7(3), 115-117. doi: 10.11648/j.ijmb.20220703.11
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Download@article{10.11648/j.ijmb.20220703.11,
author = {Dancun King’ang’i Muchira and Elias Mwangi and Richard Odour},
title = {Optimized Nematodes Isolation Protocol for Soils from Tissue Culture Banana Field},
journal = {International Journal of Microbiology and Biotechnology},
volume = {7},
number = {3},
pages = {115-117},
doi = {10.11648/j.ijmb.20220703.11},
url = {https://doi.org/10.11648/j.ijmb.20220703.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220703.11},
abstract = {Nematodes are one of the most economically important pests affecting banana production worldwide. Studies on how nematodes affect banana production involve isolating the nematodes from the banana fields for further analysis laboratory. Different protocols have been developed however, the protocols have not been fine-tuned to save on time and increase efficiency. The time required for isolation therefore needs to be optimized so that the optimal number of nematodes is isolated. This study reports optimization of nematodes isolation time from soil samples in a tissue culture banana plantation at 5 and 9 months. The nematodes harvesting experiments were set at different times (12, 20, 24, 30, 35 and 48 hours). It also reports the influence of harvesting time at 5 and 9 months when bananas were growing in the field. The study recorded the highest number of nematodes at 48 hours which was significantly different from all the other hours evaluated. It also found that harvesting time trends at 5 and 9 months did not have significant differences. The results imply that laboratories isolating nematodes from soils where tissue culture bananas are planted can use 48 hours as optimal time for extraction of nematodes.},
year = {2022}
}
TY - JOUR T1 - Optimized Nematodes Isolation Protocol for Soils from Tissue Culture Banana Field AU - Dancun King’ang’i Muchira AU - Elias Mwangi AU - Richard Odour Y1 - 2022/08/05 PY - 2022 N1 - https://doi.org/10.11648/j.ijmb.20220703.11 DO - 10.11648/j.ijmb.20220703.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 115 EP - 117 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20220703.11 AB - Nematodes are one of the most economically important pests affecting banana production worldwide. Studies on how nematodes affect banana production involve isolating the nematodes from the banana fields for further analysis laboratory. Different protocols have been developed however, the protocols have not been fine-tuned to save on time and increase efficiency. The time required for isolation therefore needs to be optimized so that the optimal number of nematodes is isolated. This study reports optimization of nematodes isolation time from soil samples in a tissue culture banana plantation at 5 and 9 months. The nematodes harvesting experiments were set at different times (12, 20, 24, 30, 35 and 48 hours). It also reports the influence of harvesting time at 5 and 9 months when bananas were growing in the field. The study recorded the highest number of nematodes at 48 hours which was significantly different from all the other hours evaluated. It also found that harvesting time trends at 5 and 9 months did not have significant differences. The results imply that laboratories isolating nematodes from soils where tissue culture bananas are planted can use 48 hours as optimal time for extraction of nematodes. VL - 7 IS - 3 ER -
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